Get Novel Chemotherapeutic Agents: Preactivation in the PDF

By Kirpal S. Gulliya

ISBN-10: 3662222418

ISBN-13: 9783662222416

ISBN-10: 3662222434

ISBN-13: 9783662222430

This publication is set a discovery termed "preactivation" that's thought of an enormous step forward in photodynamic treatment and in drug improvement. the method of preactivation generates formerly unknown chemotherapeutic brokers that aren't in simple terms powerful opposed to malignancies and viral infections, yet also are simply tolerated.

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Extra info for Novel Chemotherapeutic Agents: Preactivation in the Treatment of Cancer and AIDS

Example text

LM). Reprinted with permisssion from Photochemistry and Photobiology 1990; 2:831-838. 34 Novel Chemotherapeutic Agents Fig. 9 JlM)]. Viability of cells was determined by trypan blue dye exclusion method on indicated days. Next, effects of pMC540 on the colonogenic growth of cultured tumor cells from different cell lines were also determined by using a colony formation assay. For this purpose cells were treated with either pMC540, non-activated MC540, or left untreated. 6 mM 2-mercaptoethanol and 100 units of penicillin/100 Jlg streptomycin.

4 u c ftl ... 2 \ \ \ ' ........ ~--r---~-------,--------~-~-~~--~r--- 200 250 300 350 400 450 500 540 600 Wavelength (nm) Fig. 2. 7Absorbance spectrum of native MC540 and preactivated MC540 (pMC540) in 70% ethanol:water. The absorbance spectrum measurements were carried out using a Perkin-Elmer spectrophotometer. Results show that absorbance maxima of preactivated MC540 are no longer in the region of native MC540. There is increased absorption at 280 nm by preactivated MC540, compared to that of native MC540.

2 \ \ \ ' ........ ~--r---~-------,--------~-~-~~--~r--- 200 250 300 350 400 450 500 540 600 Wavelength (nm) Fig. 2. 7Absorbance spectrum of native MC540 and preactivated MC540 (pMC540) in 70% ethanol:water. The absorbance spectrum measurements were carried out using a Perkin-Elmer spectrophotometer. Results show that absorbance maxima of preactivated MC540 are no longer in the region of native MC540. There is increased absorption at 280 nm by preactivated MC540, compared to that of native MC540.

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Novel Chemotherapeutic Agents: Preactivation in the Treatment of Cancer and AIDS by Kirpal S. Gulliya


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