Read e-book online Molecular of Cloning of Recombinant DNA PDF

By W.A. Scott (Eds.)

ISBN-10: 0126342504

ISBN-13: 9780126342505

ISBN-10: 0323158722

ISBN-13: 9780323158725

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Extra resources for Molecular of Cloning of Recombinant DNA

Example text

In particular, the Hindlll cleavage site adjacent to the structural gene for tetracycline resistance in the pSClOl plasmid and the HindIII cleavage site in the kanamycin operon of pSC105 and its derivatives appear to be located between promoters from structural genes (Chang and Cohen, unpublished data; H. Boyer, personal communication; A. Rambach, personal communication; ref. 22). In certain instances, insertion of a DNA fragment into such cleavage sites blocks expression of kanamycin and/or tetracycline resistance while insertion of other fragments permits expression to occur.

The reasons for this are not clear; it may be simply that the extrachromosomal state of plasmids provides more opportunity for genome-genome interaction, but we don't know that.

Timmis, Proceedings of the Miles Symposium on Recombinant DNA (1977). S. Ν. COHEN et al. K. Timmis, F. N. Cohen, Proc. Natl. Acad. , 71 (1974) 4556-4560. A. Rambach and P. Tiollais, Proc. Natl. Acad. , 71 (1974) 3927-3930. N . E . Murray and K. Murray, Nature, 251 (1974) 476-481. M. R. W. Davis, Proc. Natl. Acad. , 71 (1974) 4579-4583. E. Lobban and A . D . Kaiser, J. M o l . , 78 (1973) 453-471. A. H. Symons and P. Berg, Proc. Natl. Acad. , 69 (1972) 2904-2909. L. Clarke and J. Carbon, Proc. N a t l .

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Molecular of Cloning of Recombinant DNA by W.A. Scott (Eds.)


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