By Nagina Parmar, Ajay Singh
Over the prior four billion years, microorganisms have assisted in shaping the earth and making it extra liveable for greater types of lifestyles. they're notable of their metabolic variety and their skill to reap power from oxidation and aid reactions. examine on those microbiological tactics has resulted in the newly evolving fields of geomicrobiology and biogeochemistry, linking the geosphere and the biosphere.
This quantity of the Soil Biology sequence presents an summary of the biogeochemical tactics and the microorganisms concerned, with an emphasis at the business functions. subject matters taken care of contain points resembling bioremediation of infected environments, biomining, biotechnological purposes of extremophiles, subsurface petroleum microbiology, stronger oil restoration utilizing microbes and their items, steel extraction from soil, soil elemental biking and plant nutrition.
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Extra resources for Geomicrobiology and Biogeochemistry
Microarrays have originally been developed for gene expression analysis of individual organisms or pure cultures and their use with environmental samples bears special challenges (Zhou and Thompson 2002; Gentry et al. 2006). Some difficulties are associated with the sample matrix, such as the presence of substances like humic acids, metals, and organic contaminants which can interfere with the hybridization step, or the fact that some environments contain only low levels of 2 Novel Molecular Tools to Assess Microbial Activity in Contaminated Environments 25 biomass creating the need to amplify nucleic acids, which as mentioned before may introduce bias.
Microbiol Mol Biol Rev 59:143–169 Avaniss-Aghajani E, Jones K, Chapman D, Brunk C (1994) A molecular technique for identification of bacteria using small subunit ribosomal RNA sequences. Biotechniques 17:144–146 Bae S, Wuertz S (2009) Discrimination of viable and dead fecal bacteroidales bacteria by quantitative PCR with propidium monoazide. Appl Environ Microbiol 75:2940–2944 Baldwin AJ, Moss JA, Pakulski JD, Catala P, Joux F, Jeffrey WH (2005) Microbial diversity in a Pacific Ocean transect from the Arctic to Antarctic circles.
2011) were able to find close associations of the four most dominant operational taxonomic units detected in T-RFLP to phylum or genus level, when analyzing spatial and temporal variations of the microbial community in a tailings basin of a Pb–Zn mine. Different studies have used DGGE/TGGE and T-RFLP to assess microbial community composition in different contaminated environments. Spatial and temporal variations of microbial community composition were analyzed in different mining environments such as an acidic stream draining across a pyrite mine in China (Tan et al.
Geomicrobiology and Biogeochemistry by Nagina Parmar, Ajay Singh