By Jiang Gu
This quantity is a suite of chapters contributed through top specialists within the rising box of analytical morphology. Its contents conceal a variety of strategies for morphologic examine and analysis and it really is meant for somebody who desires to retain abreast of the speedy improvement during this box. Analytical morphology is a latest technological know-how facing the analy sis of form, measurement and colour association of mobile and tissue parts by way of a number of analytical maneuvers. It differs from traditional mor phology and histopathology in that it employs equipment past regimen hema toxylin and eosin or histochemical staining. To a superb quantity, the development ment of analytical morphology relies on new advances in different disciplines, comparable to immunology, molecular biology, laser know-how, microwave tech nology and computing device technological know-how. utilizing those new equipment, specific mobile elements that will differently stay invisible, akin to peptides, professional teins, or nucleotide sequences, are highlighted through obvious markers via chemical, actual, immunological or molecular organic reactions. those equipment comprise immunocytochemistry, in situ hybridization, in situ poly merase chain response, antigen retrieval, picture research, and so forth. Analytical morphology is the basis of up to date drugs. It presents concrete and visual proof for plenty of conceptual deductions of different lifestyles technological know-how disciplines.
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Additional info for Analytical Morphology: Theory, Applications and Protocols
Gasc. 1995. Enhancement of mRNA in situ hybridization signal by microwave heating. Lab. Invest. 73:586-591. -P. J. Isola. 1993. Proliferating cell nuclear antigen immunohistochemistry using monoclonal antibody 19A2 and a new antigen retrieval technique has prognostic impact in archival paraffin-embedded node-negative breast cancer. Am. J. Pathol. /42:1081-1089. , I. Leivo, K. von Boguslawsky and E. Saksela. 1994. Cell proliferation correlates with prognosis in acinic cell carcinomas of salivary gland origin.
6) For IHC staining, follow the same procedure as for MW heating. 6. Pressure cooking method (134) (1) A domestic cooker with an operating pressure of 103 kPa/15 psi is onethird filled with AR solution and heated by hotplate to boil. (2) Slides suspended in metal slide racks are placed quickly into the AR solution, and the pressure lid is tightly replaced. (3) Timer starts when the pressure indicator valve reaches the maximum (around 4 min). The optimal period of pressurized boiling is 1-2 min.
Lab. Invest. 57:230-236. -H. J. Heidebrecht, K. Haas, M. Hauberg, M. Tiemann and R. Parwaresch. 1993. Determination of the growth fraction in non-Hodgkin's lymphomas by monoclonal antibody Ki-S5 directed against a formalin-resistant epitope of the Ki-67 antigen. Am. J. Pathol. 142:1689-1694. , H. W. Tilanus, M. Van Blankenstein, F. T. H. Mulder. 1995. Accumulation of p53 protein in normal, dysplastic, and neoplastic barrett's oesophagus. J. Pathol. 175:175-180. , G. Key, M. Duchrow, C. -D. Flad and J.
Analytical Morphology: Theory, Applications and Protocols by Jiang Gu