By R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. Dr. Ralf Oelmüller (eds.)
"Advanced recommendations in Soil Microbiology" provides quite a lot of biotechnological equipment for program in soil microbiology research. those comprise all crucial equipment concerning molecular biology, immunology, microbiology, and structural biology, reminiscent of transcriptome research, RNAi expertise, molecular matchmaking, RAPD, T-RFLP and FT/MS.
The recommendations and methods were chosen with the purpose of delivering functional publications for instant use within the laboratory. The structures investigated diversity from person molecules and cells to whole eukaryotic organisms, with a spotlight on micro organism, fungi, mycorrhiza, and better vegetation. This quantity of cutting-edge, perform orientated equipment can be of serious use either to the first-timer and to the skilled scientist.
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Extra info for Advanced Techniques in Soil Microbiology
Molecular Phylogenet Evol 27:372–383 Jeewon R, Liew ECY, Hyde KD (2003b) Molecular systematics of the Amphisphaeriaceae based on cladistic analyses of partial LSU rDNA sequences. Mycol Res 107:1392–1402 Jeewon R, Liew ECY, Hyde KD (2004) Phylogenetic evaluation of species nomenclature of Pestalotiopsis in relation to host association. Fungal Divers 17:39–55 Kirk JL, Beaudette LA, Hart M, Moutoglis P (2004) Methods of studying soil microbial diversity. J Microbiol Methods 58:169–188 Kouichi S, Yoshihisa S, Masanori S, Kazuo S (2005) A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction-denature gradient gel electrophoresis.
Grassl Sci 51:179–181 Kowalchuk GA, Gerards S, Woldendorp JW (1997) Detection and characterization of fungal infections of Ammophila arenaria (Marram grass) roots by denaturing gradient gel electrophoresis of specifically amplified 18s rDNA. Appl Environ Microbiol 63:3858–3865 Landeweert R, Hoffland E, Finlay RD, Kuyper TW, Breemen van N (2001) Linking plants to rocks: ectomycorrhizal fungi mobilise nutrients from minerals. Trends Ecol Evol 16:248–254 Laurent FM, Philippot L, Hallet S, Chaussod R, Germon JC, Soulas G, Catroux G (2001) DNA extraction from soils: old bias for new microbial diversity analysis methods.
Similar strategies are required for fungi. However, there is insufficient knowledge on the nutritional and environmental demands of soil fungi and these present methodological drawbacks in providing a clear assessment of fungal communities associated with soil. Another major complication with cultural studies is that a large number of other fungi existing as mycelial (vegetative) propagules or dormant spores can be numerically dominant populations in their natural environment but never grow in culture.
Advanced Techniques in Soil Microbiology by R. Jeewon, K. D. Hyde (auth.), Prof. Dr. Ajit Varma, Prof. Dr. Ralf Oelmüller (eds.)